aavs  (Jackson Laboratory)

Journal: Molecular Therapy. Nucleic Acids

Article Title: Orthogonal characterization of rAAV reveals vector attributes that drive ITR repair, self-complementary genome formation, and transgene expression

doi: 10.1016/j.omtn.2026.102899

Figure Lengend Snippet: Recombinant AAV genome element and heterogeneity analysis by native and alkaline electrophoresis (A) Graphic representation of the main recombinant AAV vector genome elements evaluated: wtITR (143 nt, black) versus dITR/wtITR (11-nt deletion in the 5′ C arm, 130 nt, red), EAlbAAT (albumin enhancer-alpha1-antitrypsin promoter, blue), Egfp (enhanced green fluorescent protein, green), pA (bovine/human growth hormone polyadenylation signal, yellow), and CBA (CMV (cytomegalovirus) enhancer-chicken beta actin promoter, orange). Vector genome sizes are shown next to each representation. (B) Native automated electrophoresis of extracted rAAV genomes from dITR/wtITR and wtITR. EalbAAT . Egfp vectors by TapeStation. From left to right: AAV2, AAV3B, AAV5, AAV8, AAV9, and AAVLK03 serotypes. As a run reference, the upper marker (10,000 bp) and lower marker (15 bp) of the ladder are highlighted in purple and green, respectively. (C) Native automated electrophoresis of extracted rAAV genomes from AAV5 and AAV8 serotypes with dITR vs. wtITR and CBA versus EalbAAT promoters. As a run reference, the upper marker (10,000 bp) and lower marker (15 bp) of the ladder are highlighted in purple and green, respectively. (D) Native automated electrophoresis of extracted rAAV genomes from the AAVLK03 serotype with dITR and CBA vs. EalbAAT promoters. As a run reference, the upper marker (10,000 bp) and lower marker (15 bp) of the ladder are highlighted in purple and green, respectively. (E) Native electrophoresis of extracted rAAV genomes from AAV9 and AAVLK03 serotypes with dITR and CBA versus EalbAAT promoters, before and after digestion at 37°C for 1 h with SmaI. (F) Graphic representation of the dITR/tITR design (11-nt deletion in the 5′ C arm and 22-nt deletion of the 3′ B arm, 121 nt, purple), scITR (C arm deletion, 106 nt, green), and the dITR. AAT . Egfp expression cassette including an hypoxanthine phosphoribosyltransferase 1 ( HPRT 1 ) DNA stuffer sequence (GenBank: NG_012329.2 ) (bottom). Vector genome sizes are shown next to each design. (G) Alkaline electrophoresis of AAV8 vectors with different ITR configurations and CBA versus EalbAAT promoters. (H) Alkaline electrophoresis of AAV8 and AAV3B vectors with dITR/wtITR, dITR/tITR, and scITR configurations. (I) Alkaline electrophoresis of AAV8 and AAVLK03 vectors with the dITR/tITR configuration. (J) Alkaline electrophoresis of AAV3B, AAV5, and AAVLK03 vectors with dITR/wtITR configuration and CBA versus EalbAAT promoters. Combinations are displayed on top of each lane. Agarose gel ladder: FastGene 1 kb DNA marker (10,000–100 bp).

Article Snippet: For the SMN1 vectors, AAV genome integrity was analyzed using the AAV sequencing service provided by Plasmidsaurus Inc. (Eugene, OR, USA).

Techniques: Recombinant, Electrophoresis, Plasmid Preparation, Marker, Expressing, Sequencing, Agarose Gel Electrophoresis

Journal: Molecular Therapy. Nucleic Acids

Article Title: Orthogonal characterization of rAAV reveals vector attributes that drive ITR repair, self-complementary genome formation, and transgene expression

doi: 10.1016/j.omtn.2026.102899

Figure Lengend Snippet: Recombinant AAV genome element effect and heterogeneity analysis by long-read single molecule, real-time SMRT sequencing (A) Read counts and length of rAAV3B and rAAVLK03 vector genomes encoding the Egfp transgene with dITR, dITR/tITR, and scITR designs, and AAT versus CBA promoters. (B) Identification of vector genome start and end sequencing positions from the AAV3B and AAVLK03 constructs (displayed on top). Start positions are labeled in blue, and end positions are labeled in red. Quantification of read counts is displayed in the Y axis. Vector genome elements are displayed on top as a reference. (C) Read counts and length of rAAV8 vector genomes encoding the Egfp reporter transgene with different ITR designs (from left to right: wtITR, dITR/wtITR, dITR/tITR, and scITR) under the control of the EAlbAAT or CBA promoters. The dITR.AAT. Egfp . Stuffer vector is also included. (D) Identification of vector genome start and end sequencing positions from the aforementioned AAV8 constructs. Start positions are labeled in blue, and end positions are labeled in red. Quantification of read counts is displayed on the Y axis. Vector genome elements are displayed on top as a reference.

Article Snippet: For the SMN1 vectors, AAV genome integrity was analyzed using the AAV sequencing service provided by Plasmidsaurus Inc. (Eugene, OR, USA).

Techniques: Recombinant, Sequencing, Plasmid Preparation, Construct, Labeling, Control

Journal: Molecular Therapy Advances

Article Title: Continuous production of recombinant adeno-associated virus in the insect cell/baculovirus expression vector system

doi: 10.1016/j.omta.2026.201744

Figure Lengend Snippet: Analysis of genetic stability of rBV encoding rAAV genes through serial passages (A) Experimental layout of serial passage for rBV in Sf9 cells. Cell culture supernatant containing the budded rBV is subcultured into a new flask containing uninfected Sf9 cells every 72 h. (B–E) Quantification of baculovirus and AAV proteins. (B) and (D) Total budded BV and rBV were quantified from cell supernatant using ddPCR. (C) and (E) Capsid and Rep proteins were quantified from cell suspension using ELISA.

Article Snippet: Cell lysates were used for the quantification of total Rep proteins using AAV Rep ELISA kit (Cell Biolabs, California, USA) according to the manufacturer’s protocol.

Techniques: Cell Culture, Suspension, Enzyme-linked Immunosorbent Assay

Journal: Molecular Therapy Advances

Article Title: Continuous production of recombinant adeno-associated virus in the insect cell/baculovirus expression vector system

doi: 10.1016/j.omta.2026.201744

Figure Lengend Snippet: Continuous rAAV production using a three-tank cascade (A) Total BVs and rBVs were quantified from cell culture supernatant using ddPCR with primers to target the lef2 and AAV genes (B) rAAV genome titer was quantified from cell suspension quantified using ddPCR. (C) Quantification of total AAV capsid produced during the continuous production from cell suspension (D) Rep concentration from cell lysates using ELISA (E) SYPRO Ruby staining of denatured AAV capsids produced from the continuous production showing viral proteins (VPs). (F) Infectivity of budded rBV stored in spent media for 3 months at 4°C. The budded rBVs collected at different time points were used to infect Sf9 cells to produce rAAV.

Article Snippet: Cell lysates were used for the quantification of total Rep proteins using AAV Rep ELISA kit (Cell Biolabs, California, USA) according to the manufacturer’s protocol.

Techniques: Cell Culture, Suspension, Produced, Concentration Assay, Enzyme-linked Immunosorbent Assay, Staining, Infection